Laser-light scattering approach to peptide–membrane interaction

© International University Line, 2010Membrane-active peptides are becoming widely used, mainly due to their high therapeutic potential. Although the therapeutic action is characterized, the mechanisms of interaction are often unclear or controversial. In biophysical studies, non-invasive techniques are overlooked when studying the effect of peptides on membranes. Light scattering techniques, such as dynamic light scattering and static light scattering, can be used as tools to determine whether promotion of membrane aggregation in the presence of peptides and of self-peptide aggregation in solution occurs. More recently, light scattering has been used for evaluating the alteration on membrane surface charge (ζ-potential) promoted by membrane–peptide interactions. The data obtained by these techniques (either by themselves or combined with complementary experimental approaches) therefore yield valuable elucidations of membrane-active peptides’ mechanisms of action at the molecular level.This work was partially supported by the Fundação para a Ciência e Tecnologia (FCT) of the Portuguese Ministry of Science, Technology and Higher Education. M.M.D. acknowledges the grant SFRH/BD/41750/2007 from FCT

A "diamond-ring" star: the unusual morphologic structure of a young (multiple?) object

We have observed IRAS06468-0325 obtaining optical and infrared images through IJHKs and L' filters, K-band low-resolution spectroscopy, together with millimetre line observations of CO and CS. IRAS06468-0325 has a very unusual and enigmatic morphology with two components: a bright, close to point-like source (the diamond) and a sharp-edge ring-like structure (the ring). The source is not detected in the optical, at wavelengths shorter than the I-band. The diamond is seen in all the imaging bands observed. The ring-like structure in IRAS06468-0325 is clearly seen in the I, J, H, and Ks. It is not detected in the L'-band image. Infrared colours of the diamond are compatible with excess circumstellar emission and a young stellar nature. A strongly non-gaussian and moderately bright CO(1-0) and {13}CO(2-1) lines are seen towards IRAS06468-0325, at v_{LSR} of 30.5 km s{-1} (corresponding to a kinematic distance of 3 kpc). Very weak C{18}O(2-1) and CS(2-1) lines were detected. K-band spectra of the diamond and of the ring are similar both in the slope of the continuum and in the presence of lines supporting the idea that the ring is reflected light from the diamond. With the current data, a few different scenarios are possible to explain the morphology of this object. However, the available data seem to favour that the morphology of IRAS06468-0325 correspond to a young stellar multiple system in a transient stage where a binary co-exists with a circumbinary disc, similar to the case of GG Tau. In this case, the sharpness of the well-defined ring may be due to tidal truncation from dynamic interactions between components in a binary or multiple stellar system. IRAS06468-0325 may be an important rare case that illustrates a short-lived stage of the process of binary or multiple star formation.Comment: 7 pages, 6 figure

Spectroscopic direct detection of reflected light from extra-solar planets

At optical wavelengths, an exoplanet's signature is essentially reflected light from the host star - several orders of magnitude fainter. Since it is superimposed on the star spectrum its detection has been a difficult observational challenge. However, the development of a new generation of instruments like ESPRESSO and next generation telescopes like the E-ELT put us in a privileged position to detect these planets' reflected light as we will have access to extremely high signal-to-noise ratio spectra. With this work, we propose an alternative approach for the direct detection of the reflected light of an exoplanet. We simulated observations with ESPRESSO@VLT and HIRES@E-ELT of several star+planet systems, encompassing 10h of the most favourable orbital phases. To the simulated spectra we applied the Cross Correlation Function to operate in a much higher signal-to-noise ratio domain than when compared with the spectra. The use of the Cross-Correlation Function permitted us to recover the simulated the planet signals at a level above 3 \sigma_{noise} significance on several prototypical (e.g., Neptune type planet with a 2 days orbit with the VLT at 4.4 \sigma_{noise} significance) and real planetary systems (e.g., 55 Cnc e with the E-ELT at 4.9 \sigma_{noise} significance). Even by using a more pessimistic approach to the noise level estimation, where systematics in the spectra increase the noise 2-3 times, the detection of the reflected light from large close-orbit planets is possible. We have also shown that this kind of study is currently within reach of current instruments and telescopes (e.g., 51 Peg b with the VLT at 5.2 \sigma_{noise} significance), although at the limit of their capabilities.Comment: Accepted for Publication on MNRAS: 2013 August 29; Online Article: http://mnras.oxfordjournals.org/content/early/2013/09/27/mnras.stt1642; 5 Figures, 11 page

Detailed theoretical models for extra-solar planet-host stars: The "red stragglers" HD37124 and HD46375

In this paper we analyse and discuss the HR Diagram position of two extra-solar planet-host stars - HD37124 and HD46375 - by means of theoretical stellar evolution models. This work was triggered by the results obtained by Laws et al. (2003) who found that these stars were in contradiction to the expectation based on their high metallicity. Fixing the age of both stars with the value based on their chromospheric activity levels and computing our own evolutionary models using the CESAM code, we are able to reproduce the observed luminosity, effective temperature and metallicity of both stars for a set of stellar parameters that are astrophysically reliable even if it is non-trivial to interpret the absolute values for these parameters. Our results are discussed in the context of the stellar properties of low mass stars.Comment: 6 pages, 2 figures, A&A, in pres

CO2 conversion to phenyl isocyanates by uranium(vi) bis(imido) complexes.

Uranium(vi) trans-bis(imido) complexes [U(κ4-{(tBu2ArO)2Me2-cyclam})(NPh)(NPhR)] react with CO2 to eliminate phenyl isocyanates and afford uranium(vi) trans-[O[double bond, length as m-dash]U[double bond, length as m-dash]NR]2+ complexes, including [U(κ4-{(tBu2ArO)2Me2-cyclam})(NPh)(O)] that was crystallographically characterized. DFT studies indicate that the reaction proceeds by endergonic formation of a cycloaddition intermediate; the secondary reaction to form a dioxo uranyl complex is both thermodynamically and kinetically hindered

Environmental typology of rivers from the Brazilian semiarid as a first step for the application of the index of biotic integrity: The case of the Chapada Diamantina

Defining environmental river types is an essential step in the development of accurate fish‐based methods (IBI, Index of Biotic Integrity) to assess the environmental quality of aquatic ecosystems. In this study, the environmental typology of the rivers and streams in the region of Chapada Diamantina was developed. Thirty‐five sampling sites representative of the upper Paraguaçu River and its main tributaries were characterized to characterize the fish assemblages and abiotic environmental descriptors. A cluster analysis based on fish species CPUE was performed to define a first biological typology. Then, a discriminant analysis model was developed to select the environmental descriptors that explained the fish‐based river types. The model selected 11 environmental variables and classified 91% of the cases. The river typology defined in this study will be used for the development of an IBI to assess the ecological status of the Chapada Diamantina rivers. It is expected that both the typology developed here and the future IBI will provide important and useful tools to develop and apply nature conservation‐oriented management schemes in the Chapada Diamantina aquatic ecosystems.info:eu-repo/semantics/acceptedVersio

Dengue and Zika viruses : epidemiological history, potential therapies, and promising vaccines

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Dengue virus (DENV), which can lead to fatal hemorrhagic fever, affects 390 million people worldwide. The closely related Zika virus (ZIKV) causes microcephaly in newborns and Guillain-Barré syndrome in adults. Both viruses are mostly transmitted by Aedes albopictus and Aedes aegypti mosquitoes, which, due to globalization of trade and travel alongside climate change, are spreading worldwide, paving the way to DENV and ZIKV transmission and the occurrence of new epidemics. Local outbreaks have already occurred in temperate climates, even in Europe. As there are no specific treatments, these viruses are an international public health concern. Here, we analyze and discuss DENV and ZIKV outbreaks history, clinical and pathogenesis features, and modes of transmission, supplementing with information on advances on potential therapies and restraining measures. Taking advantage of the knowledge of the structure and biological function of the capsid (C) protein, a relatively conserved protein among flaviviruses, within a genus that includes DENV and ZIKV, we designed and patented a new drug lead, pep14-23 (WO2008/028939A1). It was demonstrated that it inhibits the interaction of DENV C protein with the host lipid system, a process essential for viral replication. Such an approach can be used to develop new therapies for related viruses, such as ZIKV.This research was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), and Calouste Gulbenkian Foundation (FCG, Portugal) project Science Frontiers Research Prize 2010. N.M.S. acknowledges FCT-MCTES fellowship SFRH/BD/144585/2019. I.C.M. acknowledges FCT-MCTES program “Concurso de Estímulo ao Emprego Científico” (CEECIND/01670/2017).info:eu-repo/semantics/publishedVersio